FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_hbepc_b1_mock_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_hbepc_b1_mock_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34517101
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG202350.035.58388550
TATAGGG107900.033.3250248
ATAGGGG127950.030.3097849
GGGGAGA524000.025.175374
GGGAAAA483900.025.05224622
GGGGGGT644400.023.51133314
GGGAGAG547350.022.4847955
GTTGGGA216850.020.95967319
TTATAGG97150.020.92262547
GGGGGGA668450.020.397762
GGGTTGG335400.018.70623817
CGGGTAA38200.018.68629861
AGAGCGG149700.018.6421748
GGTTGGG325550.018.5830118
GGGGGAG639650.018.1963063
GGGGGTT317500.017.98786715
GTTTGGT227050.017.93298131
TTGGGAA295250.017.91886120
GGAAAAA506500.017.85700423
TGGGGAA411500.017.72936820
Produced by FastQC (version 0.11.2)