FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_hbepc_b1_mock_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_hbepc_b1_mock_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36222758
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG194950.035.05956650
TATAGGG100500.032.4826948
ATAGGGG120050.029.45931249
GGGGAGA537600.023.8253654
GGGAAAA479950.023.33962222
GGGGGGT682900.022.19155514
GGGAGAG558700.021.3235035
GTTGGGA210200.020.03025219
GGGGGGA691400.019.6049352
TTATAGG98200.018.1339947
GGGTTGG346900.017.71805217
AGAGCGG154800.017.6946778
GGGGGAG659050.017.6279723
GGTTGGG340350.017.35926418
CGGGTAA38900.017.01921861
GGGGTTG365700.016.74005116
GGGGGTT330400.016.6798615
TTGGGAA296200.016.57981920
GTTTGGT231650.016.56424931
TGGGGAA426800.016.39847620
Produced by FastQC (version 0.11.2)