FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_hbepc_b1_6h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_hbepc_b1_6h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42457113
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG180550.021.05858450
GGGGGAT338300.013.46503934
ATAGGGG105350.012.93683749
TATAGGG82700.012.85177148
GGGGGGT1231700.012.21389714
GGGTGGG1173600.011.77094717
GGGTTAA101650.011.77062566
GGGGTGG1179400.011.568933516
TGGGGGA509450.011.54307720
GGGGGGA1045200.011.2508042
GGGGGTG1170050.011.17766315
GGGGGAG1024950.010.7738983
GGGGAGA553400.010.6166114
GAGTTAA103850.010.55839966
GGTGGGG1310300.010.42176418
GGGATGT234350.010.41105636
GGGGTGT363500.010.28168636
GGGGTTA136900.09.97363165
GTAGGGG206800.09.96068849
GGGAGAG591200.09.8046765
Produced by FastQC (version 0.11.2)