FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_hbepc_b1_6h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_hbepc_b1_6h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44229885
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG257050.035.9182350
TATAGGG134750.033.54829848
ATAGGGG159450.031.18232349
GGGGAGA693550.021.4414374
GGGAAAA556950.021.19672622
GGGAGAG698100.020.4197165
GGGGGGT1051250.020.37598614
GGGGGAT399450.020.31100734
TTATAGG125000.018.44225947
AGAGGGG544000.017.6348938
TGGGGAA533450.017.61620520
GAGAGGG531600.017.6157287
ATATAGG86250.017.52518747
GGGTTGG374150.016.94409817
GGGGGAG990200.016.9422633
GAATTAA199450.016.56190166
GGGGGTG980250.016.30284915
GGGGTGG1023800.016.24985516
GGGTGGG1048200.016.09977117
GGGGGGA1183000.015.8303612
Produced by FastQC (version 0.11.2)