FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_a549_mock_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_a549_mock_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40314564
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG177200.030.86116650
ATAGGGG133850.022.83917649
TATAGGG122450.022.77092748
GGGGGGT619600.019.64116514
GGGGAGA509850.017.8326074
GGGGGGA623650.016.6717622
GGGAAAA428950.016.25123422
GTTTGGG271800.015.5394431
GGGAGAG579500.015.1969385
GGGGGAG644800.014.334263
TTATAGG121700.014.32026647
AGGGGGG590300.014.14469751
GGGGGTG633650.013.98014415
AGAGGGG408200.013.91858
GAGAGGG410450.013.2856927
GGGTGGG729300.013.20260517
GGGTTGG302650.013.14771417
GGGGTTG305300.013.01032216
GTTGGGA195650.012.91212419
GGGGTGG737250.012.81616816
Produced by FastQC (version 0.11.2)