FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_a549_mock_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_a549_mock_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44995461
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG210750.033.0525550
TATAGGG155350.028.1894248
ATAGGGG163450.027.97039449
GGGGGGT666200.022.42650214
GGGGAGA605800.021.8548834
GGGAAAA577700.021.37928422
ATATAGG94500.019.31876847
TTATAGG151700.018.85925547
GGGGGGA701650.018.8377442
GTTGGGA243000.018.53380819
GGGAGAG666650.018.424175
GTTTGGG326500.018.10380431
GGGTTGG351100.017.5159417
GGGGTTG355950.017.34402516
GGGATAT137400.016.7022735
AGAGCGG172050.016.5819248
GGGGGAG694450.016.1640933
TATATAG101350.016.07212646
CGGGTAA45150.015.809442561
GGGGGTT352350.015.78759215
Produced by FastQC (version 0.11.2)