FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_a549_6h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_a549_6h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38048086
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG163650.035.06051350
ATAGGGG129800.028.17752849
TATAGGG127050.027.35165848
GGGGGGT527200.022.3636114
GGGGAGA474250.022.136684
GGGAAAA480000.019.93678922
GGGGGGA543050.019.4060332
GTTTGGG258750.019.28345331
GGGAGAG536150.018.3995385
GTTGGGA191450.017.32364319
CGGGTAA36250.017.1219461
AGAGCGG140350.017.0329238
GGGTTGG274700.016.98576717
GGGGGAG538800.016.7380563
GGGGTTG276800.016.64365216
TTATAGG131750.016.39748247
GGGGGTT279450.015.49756115
ATATAGG89950.015.30823747
AGGGGGG537450.015.24673151
CGCGAAA22800.014.9713061
Produced by FastQC (version 0.11.2)