FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n02_a549_6h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n02_a549_6h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37299198
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG139750.027.0336950
ATAGGGG116400.020.7791849
TATAGGG112600.020.56438348
GGGGGGT450850.018.45961414
GGGGAGA414100.017.318324
GGGGGGA454600.016.7969652
GGGAAAA406450.015.81813722
GTTTGGG230700.014.07479731
GGGAGAG490850.013.7197975
GGGGGAG497650.013.148573
GGGTTGG249350.013.07920617
GTTGGGA170100.013.00540119
AGAGCGG123450.012.9097938
AGGGGGG443450.012.728765551
TTATAGG121100.012.39647647
GGGGTTG265600.012.38230916
GGGGGTT255350.012.14943115
ATATAGG79750.011.98677247
CGGGGAT100500.011.8364241
CGCGAAA19050.011.7648341
Produced by FastQC (version 0.11.2)