FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_hbepc_b2_mock_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_hbepc_b2_mock_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37012158
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA391380.10574363159262423TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG133900.064.31010441
CGCGTAT86250.062.2417244
TATGCCG246550.061.87921548
ATCGCGT103550.061.83716642
TCGCGTA89750.061.08244343
ACACGTC630950.060.0041313
CTCGTAT224300.059.3576344
CACTCCG628750.059.23828531
CGTATGC245350.058.4835846
TCGTATG204150.058.37192545
ACGTCTG653050.057.84113715
CACACGT656050.057.69341712
CGTCTGA654800.057.67074616
ACTCCGG646650.057.65711232
CACGTCT658600.057.39584714
GATCGGA656250.056.5991021
ATCGGAA670750.056.271022
AATCTCG294350.056.1652941
GTCACTC677500.055.2504329
CCGGAGA678600.055.02352535
Produced by FastQC (version 0.11.2)