FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_hbepc_b2_mock_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_hbepc_b2_mock_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39579741
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5471021.3822778678617427TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG670950.066.02350645
CGTATGC675900.065.5400646
TATGCCG677150.065.2815148
CTCGTAT629050.064.80544
TTACTCG689450.064.2815235
TACTCGA689150.064.2595236
ACTCGAT690200.063.3767537
TCTCGTA645900.062.4569243
ACACGTC742550.060.93339513
GTATGCC728550.060.7138747
ATTACTC733150.060.5441634
ATGCCGT730600.060.542949
ATCTCGT667600.060.21962442
ACGTCTG755800.059.73727815
ACATTAC747750.059.57335732
CGTCTGA763900.059.13554816
CACGTCT764150.059.1251514
CTCGATC682100.059.000738
CCGTCTT749650.058.84799252
CACACGT771950.058.66672512
Produced by FastQC (version 0.11.2)