FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_hbepc_b1_mock_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_hbepc_b1_mock_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34517101
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3885471.125665217365734TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG468750.065.4463645
TATGCCG472100.064.9895548
CGTATGC475600.064.66334546
CTCGTAT455800.063.99046344
TCTCGTA470800.061.3212143
GTCACGA506750.061.08838729
AGTCACG516950.059.8963128
CGAGATT517750.059.61138533
TCACGAG522200.059.30125430
ATCTCGT487850.059.04242342
ATGCCGT521050.058.91008449
GTATGCC524050.058.69202847
ACGAGAT534050.057.9655432
ACACGTC542550.057.76323313
CCGTCTT535050.057.23911352
CACGAGA541750.057.23123631
ACGTCTG555750.056.29781715
GCCGTCT547200.056.01223851
CACACGT568700.055.09574512
CACGTCT568400.055.03298614
Produced by FastQC (version 0.11.2)