FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_hbepc_b1_mock_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_hbepc_b1_mock_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36222758
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4143581.1439162086995143TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG492150.065.3566345
TATGCCG496200.064.7883348
CGTATGC497200.064.7136246
CTCGTAT491050.064.0553344
TATCTCG495950.063.2322841
TCTCGTA510000.061.4986743
ATCTCGT528700.059.42037242
ATGCCGT545200.058.88246549
GTATGCC548950.058.59416247
ACCGCTC560000.057.5469632
CCGTCTT559350.057.0894952
CGCTCAT565850.056.9093434
ACACGTC583100.056.39640813
GCCGTCT573500.055.77747351
CACCGCT582200.055.4535931
ACGTCTG595500.055.21627815
TCACCGC585050.055.1954830
CATTATC569700.055.1192738
TGCCGTC583350.054.9954950
TTATCTC574800.054.61836640
Produced by FastQC (version 0.11.2)