FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_hbepc_b1_6h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_hbepc_b1_6h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42457113
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG141800.063.35453841
CGCGTAT93650.062.03847544
TATGCCG272200.061.53595448
ATCGCGT111400.061.2886742
TCGCGTA97500.061.21603843
CACTCCG681400.059.13665431
CTCGTAT242800.058.88406844
ACACGTC695400.058.6895113
TCGTATG214750.058.6229245
CGTATGC266400.058.14855646
ACGTCTG711150.057.3945515
ACTCCGG702250.057.36142332
CACGTCT718600.056.7851214
CACACGT731900.055.79132512
CGTCTGA731650.055.772416
AATCTCG322100.055.54625341
TCTCGTA267250.054.58093643
GTCACTC745900.054.23070529
CGGAGAA741150.054.2204836
CCGGAGA747800.053.8490335
Produced by FastQC (version 0.11.2)