FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_hbepc_b1_6h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_hbepc_b1_6h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44229885
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5457511.2338964932872876TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG683150.065.6940245
TATGCCG689550.064.9693648
CGTATGC692100.064.8842946
CTCGTAT637050.064.33893644
TACTCGA711000.063.02144636
TTACTCG717200.062.5055135
TCTCGTA654450.062.05336843
ACTCGAT715200.062.01440437
ATGCCGT747000.059.95315649
GTATGCC749150.059.92962347
ATCTCGT678850.059.69998642
ACACGTC775400.058.91234213
ATTACTC766500.058.61161834
ACATTAC773200.058.2773532
CTCGATC694850.058.23997538
CCGTCTT769150.058.08665552
ACGTCTG790450.057.74245515
GCCGTCT776900.057.5516851
TCGATCT711600.056.87381439
CACGTCT803800.056.80931514
Produced by FastQC (version 0.11.2)