FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_a549_mock_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_a549_mock_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40314564
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG93800.060.79712741
CGCGTAT60600.059.77905744
TATGCCG178250.057.1761648
ATCGCGT75700.056.55817442
ACACGTC449900.055.91649213
TCGCGTA65850.055.640943
CTCGTAT158850.055.01490844
CGTATGC172200.054.95739746
CACTCCG450950.054.8920931
ACGTCTG455900.054.88539515
TCGTATG143000.054.357445
CACGTCT468000.053.57699214
CACACGT473900.053.1071812
GATCGGA477450.051.7821041
ACTCCGG482000.051.39887632
ATCGGAA488950.051.347242
AATCTCG212800.051.06836341
CGTCTGA493150.050.79566616
CGGAGAA498900.049.51315736
CCGGAGA503150.049.2661135
Produced by FastQC (version 0.11.2)