FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_a549_mock_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_a549_mock_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44995461
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3790740.8424716439731553TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG474650.064.98900645
CGTATGC478400.064.4363146
TATGCCG481900.063.86120248
CTCGTAT447150.063.31477444
TACTCGA501650.061.22863436
TCTCGTA463850.060.3877543
ACTCGAT503950.060.27822537
TTACTCG515450.059.66963635
ATCTCGT484350.057.70293842
ACACGTC546400.057.52607313
GTATGCC546750.056.4505747
ACGTCTG555000.056.39225415
ATGCCGT552650.055.74110449
CACGTCT571750.054.83069614
CACACGT574400.054.6924612
ACATTAC572200.054.125232
CCGTCTT567800.054.0650452
CTCGATC517400.054.01692238
ATTACTC573050.053.84678634
GCCGTCT571650.053.73700751
Produced by FastQC (version 0.11.2)