FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_a549_6h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_a549_6h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38048086
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2989870.7858135097781266TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG370550.064.5417245
CGTATGC372850.064.19910446
CTCGTAT361300.062.71809444
TATGCCG381600.062.60037648
TCTCGTA374550.059.90003643
GTCACGA406550.059.3086329
AGTCACG409750.058.96333328
TCACGAG418550.057.6331130
ATCTCGT389300.057.55037342
ACACGTC426050.057.22490713
ACGAGAT421950.057.0375932
CGAGATT420500.057.0126833
ACGTCTG430700.056.45489515
GTATGCC428600.055.84850347
CACGAGA433550.055.7663631
ATGCCGT437650.054.6849649
CCGTCTT443000.053.6652452
CACACGT457450.053.35759412
GCCGTCT449600.052.9465851
CACGTCT460250.052.89021714
Produced by FastQC (version 0.11.2)