FastQCFastQC Report
Wed 29 Nov 2017
HWNGLBGX3_n01_a549_6h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNGLBGX3_n01_a549_6h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37299198
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2289190.6137370567592365TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG278700.063.356745
CGTATGC282850.062.46338346
CTCGTAT280400.061.56982844
TATGCCG286500.061.42683848
TATCTCG294800.058.61874441
TCTCGTA300750.057.48382643
ATCTCGT315150.054.86686742
ACACGTC333350.054.0646413
ACCGCTC330900.053.59002332
ACGTCTG338100.053.2028315
GTATGCC338650.052.2324447
ATGCCGT341850.051.61181649
CGCTCAT350500.050.59346834
GTCACCG350950.050.56770729
CACCGCT354650.050.14735831
CCGTCTT352900.049.78957752
CACACGT363700.049.591612
CACGTCT364100.049.4796714
GCCGTCT355400.049.468451
GATCGGA371750.047.7594951
Produced by FastQC (version 0.11.2)