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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

         Loading report..

        Report generated on 2018-01-25, 07:01 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/HWNG7BGX3/merged

        General Statistics

        Showing 37/37 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HWNG7BGX3_n01_kcl_control_1
        53.8%
        47%
        8.6
        HWNG7BGX3_n01_kcl_control_2
        51.7%
        47%
        9.9
        HWNG7BGX3_n01_ko0240_1
        53.9%
        47%
        11.8
        HWNG7BGX3_n01_ko060_1
        41.1%
        45%
        8.8
        HWNG7BGX3_n01_ko060_2
        37.3%
        44%
        7.5
        HWNG7BGX3_n01_ko1060_1
        45.5%
        45%
        11.6
        HWNG7BGX3_n01_ko1060_2
        43.5%
        45%
        10.4
        HWNG7BGX3_n01_ko160_1
        40.2%
        45%
        8.7
        HWNG7BGX3_n01_ko160_2
        46.2%
        45%
        12.1
        HWNG7BGX3_n01_ko6060_1
        43.7%
        45%
        10.3
        HWNG7BGX3_n01_ko6060_2
        41.1%
        44%
        9.2
        HWNG7BGX3_n01_ox0240_1
        45.6%
        45%
        12.9
        HWNG7BGX3_n01_ox0240_2
        48.8%
        45%
        13.2
        HWNG7BGX3_n01_ox060_1
        42.4%
        45%
        10.6
        HWNG7BGX3_n01_ox060_2
        42.7%
        45%
        10.2
        HWNG7BGX3_n01_ox10240_1
        43.9%
        45%
        9.9
        HWNG7BGX3_n01_ox10240_2
        48.5%
        46%
        11.7
        HWNG7BGX3_n01_ox1060_1
        42.9%
        45%
        8.7
        HWNG7BGX3_n01_ox1060_2
        39.1%
        45%
        6.7
        HWNG7BGX3_n01_ox1240_1
        48.5%
        45%
        12.4
        HWNG7BGX3_n01_ox1240_2
        48.1%
        45%
        12.0
        HWNG7BGX3_n01_ox160_2
        42.3%
        45%
        8.6
        HWNG7BGX3_n01_ox60240_1
        45.0%
        45%
        11.3
        HWNG7BGX3_n01_ox60240_2
        48.6%
        45%
        14.0
        HWNG7BGX3_n01_ox6060_1
        42.0%
        45%
        8.9
        HWNG7BGX3_n01_ox6060_2
        41.5%
        45%
        8.8
        HWNG7BGX3_n01_wt0240_1
        42.6%
        45%
        9.5
        HWNG7BGX3_n01_wt0240_2
        43.3%
        45%
        8.8
        HWNG7BGX3_n01_wt060_2
        46.4%
        44%
        11.2
        HWNG7BGX3_n01_wt10240_1
        43.3%
        45%
        10.1
        HWNG7BGX3_n01_wt10240_2
        42.4%
        45%
        8.6
        HWNG7BGX3_n01_wt1060_1
        43.4%
        45%
        9.5
        HWNG7BGX3_n01_wt1060_2
        43.7%
        44%
        8.9
        HWNG7BGX3_n01_wt1240_1
        46.9%
        45%
        10.6
        HWNG7BGX3_n01_wt1240_2
        44.5%
        45%
        9.9
        HWNG7BGX3_n01_wt160_1
        44.3%
        45%
        10.6
        HWNG7BGX3_n01_wt160_2
        39.6%
        44%
        7.6

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF ReadsUndetermined % PhiX Aligned
        4.0
        403933778
        382837960
        2.3
        1.4

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 37/37 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        ox1240_1
        12389203
        3.3
        99.0
        wt160_2
        7550384
        2.0
        99.1
        ox060_1
        10585466
        2.8
        99.1
        ko160_2
        12063248
        3.2
        99.2
        ko1060_2
        10382921
        2.8
        99.0
        wt060_2
        11204216
        3.0
        99.0
        wt10240_1
        10054316
        2.7
        99.0
        kcl_control_1
        8590879
        2.3
        99.0
        wt1240_1
        10550701
        2.8
        99.2
        wt10240_2
        8556390
        2.3
        99.0
        ox6060_2
        8792267
        2.4
        99.0
        wt1060_1
        9529806
        2.5
        98.2
        kcl_control_2
        9934042
        2.7
        98.9
        wt1240_2
        9921115
        2.7
        99.1
        ox160_2
        8562778
        2.3
        99.3
        wt0240_2
        8754940
        2.3
        99.2
        ko060_2
        7494894
        2.0
        99.0
        ko6060_2
        9211984
        2.5
        99.2
        ox60240_2
        13998960
        3.7
        99.1
        ko1060_1
        11609221
        3.1
        99.1
        ox10240_1
        9860945
        2.6
        99.0
        wt1060_2
        8882721
        2.4
        99.2
        ox60240_1
        11294773
        3.0
        99.0
        ox10240_2
        11740435
        3.1
        99.1
        ox1060_1
        8691327
        2.3
        99.0
        ko060_1
        8829590
        2.4
        99.0
        ox6060_1
        8861139
        2.4
        99.0
        ko6060_1
        10333030
        2.8
        99.0
        ox1240_2
        12029855
        3.2
        98.8
        ox060_2
        10222624
        2.7
        99.0
        ox0240_2
        13191834
        3.5
        98.9
        wt0240_1
        9503303
        2.5
        99.1
        ko0240_1
        11796719
        3.2
        99.2
        ko160_1
        8692879
        2.3
        98.8
        ox0240_1
        12853202
        3.4
        99.1
        wt160_1
        10638300
        2.8
        99.1
        ox1060_2
        6702275
        1.8
        99.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        loading..

        Sequence Length Distribution

        All samples have sequences of a single length (75bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        37 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        loading..

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.