FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7550384
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG250150.33130765269686946TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35900.052.27746646
CGTATGC37900.049.6097944
GTATGCC39100.047.9990145
ATGCCGT40950.045.74630447
TCGTATG42150.044.6076243
TAGGCAT42600.043.32771335
CTCGTAT43500.042.6683642
GCCGTCT44600.042.2345549
CACTTAG45200.041.82850631
TGCCGTC46650.040.15672348
TTAGGCA47300.039.53378334
CATCTCG46800.039.0702239
GCACACG50700.038.99083311
ACTTAGG48600.038.90223332
ACGTCTG51600.038.44473615
CACACGT52250.037.83466712
TCTCGTA49400.036.9438141
GGCATCT49400.036.87446237
ACACGTC54550.036.42917313
CACGTCT57150.034.9524914
Produced by FastQC (version 0.11.2)