FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10638300
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG456210.4288373142325372TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG64000.054.7139446
CGTATGC64000.054.44466444
GTATGCC68100.051.5211745
ATGCCGT69450.050.51968447
TCGTATG69250.050.46655343
TATCTCG74050.046.63617739
CTCGTAT75350.046.06050542
GCCGTCT77500.045.2276449
CGATGTA78550.044.7993834
TGCCGTC78700.044.45034448
CCGATGT78900.044.4255433
GCACACG83750.043.74877511
ACGTCTG86050.042.65921415
CACACGT86100.042.51463312
TCTCGTA82750.041.7747341
CACGTCT90350.040.66713314
ACACGTC91600.040.07507713
GTATCTC87900.039.52342238
ACCGATG91550.038.51328732
GTCACCG92350.038.32962829
Produced by FastQC (version 0.11.2)