FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt1240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt1240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9921115
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG329400.33201913293011925RNA PCR Primer, Index 26 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47150.053.12141444
TATGCCG48600.051.39454746
GTATGCC50450.049.57829345
ATGCCGT53750.046.6625547
TCGTATG53550.046.38608643
CTCGTAT56100.043.72415542
TGCCGTC60700.041.4903148
GCCGTCT64250.038.87567549
CATCTCG64550.038.2141839
GCACACG67750.037.4785911
ACGTCTG68400.037.37462215
TCTCGTA66300.037.10143741
ACACGTC70600.036.3569413
CACACGT70150.036.3442612
GTCACAT72400.035.31097429
CACGTCT76450.033.5294214
CATGAGC79950.032.19169233
CAGTCAC81400.031.19473327
CACATGA84300.030.77611231
AGCACAC83450.030.75838310
Produced by FastQC (version 0.11.2)