FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt1240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt1240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10550701
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA456890.4330423163351894TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC60100.055.73830846
TATGCCG60350.055.6217448
GTATGCC64500.052.04322447
TCGTATG65500.051.14333345
ATGCCGT66250.050.61618449
CTCGTAT71200.047.0489944
TATCTCG72750.046.14163641
GCCGTCT75000.044.9411751
ACGTCTG79450.043.37972315
CACACGT82950.041.6741312
GCACACG83150.041.4909111
CACACTG83450.040.59894631
TCTCGTA82900.040.5336343
TGCCGTC84550.040.10965750
TGATATA84150.040.01408836
ACTGATA85700.039.61334
TATATCT85950.039.17628539
CACGTCT89150.038.814214
GATATAT89050.037.812337
ATATATC89650.037.79031438
Produced by FastQC (version 0.11.2)