FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt1060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt1060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8882721
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG117120.13185149010083733TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23650.040.9910446
CGTATGC24500.039.70993844
GTATGCC26900.036.4233445
ATGCCGT27900.035.11804647
TCGTATG29700.032.40887543
CTCGTAT31200.029.96614542
GCCGTCT32750.029.49598349
TGCCGTC36700.026.60336348
GCACACG39100.026.38276911
ACGTCTG38700.026.2089815
CACACGT39950.025.38978212
CACAGTG42200.023.13662133
ACACGTC45050.022.43888313
CACGTCT45300.022.16185414
TCTCGTA42500.021.67397341
CACACAG45250.021.42500931
GTGTGCG12950.021.3133188
ACACAGT46550.021.0486732
GTCACAC47550.020.96925729
TCCAGTC51300.020.24323825
Produced by FastQC (version 0.11.2)