FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt1060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt1060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9529806
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG149680.15706510709661875TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25000.042.5035744
TATGCCG24900.042.39671746
GTATGCC28300.037.54693245
ATGCCGT29800.035.4254547
TCGTATG30600.034.72514343
ACTGACC32050.033.80050332
CTCGTAT32800.031.44954542
GCCGTCT37100.028.6409249
CACTGAC38250.027.96108831
ACGTCTG41000.027.51572415
GCACACG41850.026.87428311
TGCCGTC41350.025.94747448
CACACGT43900.025.54087812
CACGTCT47550.023.79799314
ACACGTC47500.023.6051513
TCTCGTA45650.022.5212541
AGCACAC54550.021.18688410
CTGACCA51100.021.13221433
GTCACTG53400.020.73920829
CAGTCAC53300.020.7133927
Produced by FastQC (version 0.11.2)