FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt10240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt10240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8556390
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG672050.7854363814646129RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC78050.060.9100944
TATGCCG80350.059.2094946
GTATGCC83100.057.2916145
TCGTATG84000.056.63669643
ATGCCGT85950.055.4323547
CTCGTAT85250.055.11828642
GCCGTCT91150.052.4210949
ACGTCTG94750.050.68483715
TGCCGTC94300.050.67001748
GCACACG95100.050.46143311
CACACGT97500.049.07834212
TCTCGTA96750.048.24639541
ACACGTC100200.047.8591613
AGTCACC103100.046.58072328
CACGTCT103400.046.44449214
CAGTCAC107650.044.54729527
AGCACAC108550.044.46321510
TCCAGTC107900.044.41210625
GATCTCG105750.043.94431339
CCAGTCA110900.043.36624526
Produced by FastQC (version 0.11.2)