FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt10240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt10240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10054316
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA348180.34629904212280577TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47400.055.0978346
TATGCCG49700.052.61718848
GTATGCC52650.049.66902547
TCGTATG55300.047.4138745
ATGCCGT55650.046.80546649
CTCGTAT58750.044.45340344
TATCTCG60000.043.12478641
GCCGTCT63000.041.56366751
TGCCGTC66050.039.80107550
GCACACG70150.038.0650111
ACGTCTG70450.037.7068515
CCTTTAT70200.037.05537437
CACACGT71950.036.96887612
TCTCGTA70850.036.7154243
ACACGTC75950.035.1128913
GTCACAT76700.034.6354629
CACGTCT78450.033.99358714
CAGTCAC84850.031.55230927
TCCAGTC85650.030.9756425
CCGTCTT85500.030.82745752
Produced by FastQC (version 0.11.2)