FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11204216
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG356320.3180231441450254TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC50350.053.37655344
TATGCCG50550.052.9603946
GTATGCC53750.050.0643645
TCGTATG56150.047.80158643
ATGCCGT56650.047.31836747
CTCGTAT59600.043.64531342
GCCGTCT64150.042.05510749
GCACACG68600.040.8865911
ACGTCTG68800.040.56733715
CACGTCT72550.038.70823314
TGCCGTC71250.038.1548748
CACACGT73900.037.81471612
TCTCGTA69350.037.06160741
ACACGTC76250.036.60403413
GTCACAT78000.034.85456529
AGCACAC82900.033.9589310
TCCAGTC83250.033.27771425
CACGATC78250.032.84614636
CAGTCAC83100.032.75640527
ACGATCT79500.032.5032837
Produced by FastQC (version 0.11.2)