FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt0240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt0240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8754940
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG413270.47204206996278675RNA PCR Primer, Index 24 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53950.054.67504546
CGTATGC54450.054.55314344
GTATGCC57950.051.079745
ATGCCGT59750.049.48287647
TCGTATG60250.049.24429343
CGGTAGC62100.047.66643533
CTCGTAT64000.045.71201742
GTCACGG66100.045.0953529
AGTCACG66550.045.04962528
GCCGTCT66050.044.86755449
GGTAGCA66150.044.69592734
ACGGTAG66700.044.5859932
CACGGTA68450.043.64770531
TGCCGTC68350.043.61011548
GTAGCAT68250.043.01712835
GCACACG70100.042.6205811
ACGTCTG70350.042.27319715
TCTCGTA69850.041.68602841
CACACGT72400.041.4095612
ACACGTC73700.040.58551413
Produced by FastQC (version 0.11.2)