FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_wt0240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_wt0240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9503303
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA118930.12514596240906978TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23250.039.4704146
TATGCCG23850.038.33259248
TCGTATG27150.034.05476845
GTATGCC27600.033.49952747
ATGCCGT30600.030.10235649
CTCGTAT32300.028.83860444
AGTCACG34800.027.26369528
TATCTCG34300.026.75486641
ACGAGTG36400.025.68561732
CACGAGT38200.024.74650631
CGAGTGG37850.024.70175633
GCCGTCT38400.024.43702751
GTCACGA38750.024.39539129
TGCCGTC38800.024.18497350
ACGTCTG41650.022.94469615
GCACACG40900.022.85957111
TCTCGTA42700.022.05706843
CACACGT43150.021.82761212
ACACGTC45450.020.72313313
GTGGATA47200.020.02757536
Produced by FastQC (version 0.11.2)