FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox6060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox6060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792267
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA152370.1733000146606103TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24550.040.7528146
TATGCCG25700.038.92946648
ATGCCGT29850.033.17043349
GTATGCC30800.032.482847
TCGTATG31700.031.77859545
CTCGTAT36000.027.79131544
GCCGTCT38050.025.93133751
GCACACG41400.025.16663611
TGCCGTC39350.025.16232350
CACACGT42300.024.46819112
ACGTCTG43900.023.89062915
TCTCGTA43400.022.9732143
CACGTCT45850.022.64869514
ACACGTC45950.022.44975713
CACAGTC50750.020.46250731
GTCACAG52800.019.60291529
ACAGTCA52800.019.60269432
CAGTCAA55200.019.31280333
AGCACAC54650.019.06471610
CCAGTCA56600.018.65223726
Produced by FastQC (version 0.11.2)