FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox6060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox6060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8861139
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG103260.11653129467893461TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20950.036.5583346
CGTATGC21500.035.62331844
GTATGCC24700.031.00815245
ATGCCGT25800.029.81948547
TCGTATG27200.027.90446343
CTCGTAT31700.024.05209242
TGCCGTC36100.022.07609448
GCCGTCT37700.020.7731349
GCACACG38850.020.60188911
CACACGT39150.020.17988612
ACGTCTG39800.020.11024915
GTGTGCG8150.019.8954058
TCTCGTA39300.019.6641641
CGGGCGC6000.018.97471215
GTCACCT42200.018.88531929
ACACGTC43500.018.3206213
CACGTCT45250.017.53564614
TGTAATC44500.017.21136536
AGCACAC49000.016.54557410
GTAATCT47700.016.27370637
Produced by FastQC (version 0.11.2)