FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox60240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox60240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13998960
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG391330.27954219456302465RNA PCR Primer, Index 36 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC58800.050.86964844
TATGCCG59300.050.20765746
GTATGCC65050.046.19407345
TCGTATG65600.045.7543543
ATGCCGT66150.045.6343747
CTCGTAT73750.039.76265742
TGCCGTC82500.036.67410348
GTCACCC83100.035.70494529
GCCGTCT86300.035.29898549
GCACACG86900.035.29371611
ACGTCTG89800.034.50020215
CACACGT89900.034.2694512
TCTCGTA87100.033.7869841
ACACGTC91150.033.76188713
CACGTCT97950.031.45302614
TCACCCA97500.030.67929830
CACCCAA98400.030.468631
CAGTCAC100450.030.32774527
AGTCACC101250.030.05415228
ACCCAAC104800.028.93702332
Produced by FastQC (version 0.11.2)