FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox60240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox60240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11294773
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG402580.3564303594237795RNA PCR Primer, Index 35 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57600.053.24711646
CGTATGC58000.052.99885644
ATGCCGT62000.049.46763247
GTATGCC62200.049.3646945
TCGTATG63450.048.3921843
TATCTCG69450.043.9630539
CTCGTAT70450.043.38818442
TGCCGTC73600.041.7652248
GCCGTCT76850.039.81939349
ACGTCTG83450.037.9103615
GCACACG82400.037.76524411
ACACGTC84050.037.1883813
TCTCGTA83450.036.835841
CACACGT86800.035.93052712
CATTTTA88000.035.3237434
CACGTCT91100.034.6131214
AGTCACC92500.033.4933928
CAGTCAC94550.033.27789727
CGTCTGA98100.032.21389816
CCAGTCA98350.031.81686826
Produced by FastQC (version 0.11.2)