FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8562778
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG206060.2406462015014286TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29700.048.32253344
TATGCCG30400.047.6635146
GTATGCC33000.043.4902845
ATGCCGT34800.041.53795247
TCGTATG35850.040.1291343
CTCGTAT41100.034.49970242
AGTCACG42950.034.21896428
GTCACGA44200.033.0170729
GCACACG46900.032.8076311
TGCCGTC44550.032.67947448
CACACGT47900.032.1951112
ACGTCTG49200.031.76515615
GCCGTCT46650.031.65209249
TCTCGTA45550.030.447641
ACACGTC51900.029.84691413
CACGTCT53400.029.07278414
CGATCAG54000.026.64190733
CAGTCAC57050.026.24563427
AGCACAC59650.025.79510310
CCAGTCA59650.025.33314726
Produced by FastQC (version 0.11.2)