FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox1240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox1240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12029855
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG137880.11461484780988633RNA PCR Primer, Index 32 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27150.037.86684844
TATGCCG27350.037.33735346
GTATGCC30600.033.7101545
TCGTATG33750.030.6662243
ATGCCGT33550.030.64298247
CTCGTAT41850.024.3187442
GCACACG48650.022.33752411
GCCGTCT46000.022.04949449
ACGTCTG50400.021.56254215
CACACGT52000.021.16411212
GTGTGCG19000.020.8812738
TGCCGTC50550.020.47422248
CGCGTAT11900.019.7169481
TCTCGTA51250.019.52182841
CCACTCA55550.019.25336533
ACACGTC57200.018.93861413
CACGTCT57900.018.82881714
CGGGCGC18300.018.09839615
CAGTCAC61000.017.36329527
AGCACAC65750.017.0003710
Produced by FastQC (version 0.11.2)