FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox1240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox1240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12389203
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG140580.1134697687978799RNA PCR Primer, Index 31 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28350.038.81970644
TATGCCG29400.037.1984546
GTATGCC33600.032.75412845
TCGTATG35550.031.34566743
ATGCCGT37200.030.04783447
CACGATA38150.028.75747134
TATCTCG43700.024.55247739
CTCGTAT44450.024.44866842
GCCGTCT47100.023.2926649
TGCCGTC51150.021.98786748
GCACACG54400.021.81630511
CGGGCGC17150.021.72589915
ACGATAT49900.021.70928435
CGCGTAT8850.021.0536041
GTGTGCG18050.020.642788
GGCGGGC18550.020.2725213
CACACGT57800.020.23454512
ACGTCTG60050.019.76348715
TCTCGTA56500.019.35659441
CCACGAT57600.019.10672833
Produced by FastQC (version 0.11.2)