FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox1060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox1060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6702275
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG208800.3115360082956906TruSeq Adapter, Index 11 (100% over 63bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC70170.1046957935924742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30400.053.22551344
TATGCCG31100.051.91658446
GTATGCC32950.049.4205145
ATGCCGT34800.046.69378747
TCGTATG34750.046.66203743
AGTCACG39450.041.45261828
CTCGTAT38700.041.09704642
ACGGCTA39800.041.00109532
CGGCTAC39900.040.72540733
GCCGTCT40650.040.31349649
GGCTACA40500.040.0368834
GCACACG43900.038.82246411
TGCCGTC42800.038.44961548
GTCACGG43150.038.13774529
ACGTCTG45650.038.0140915
CACGGCT43050.037.9063431
GCTACAT42550.037.78335635
CACACGT45900.037.1311312
CATCTCG43150.036.69858639
ACACGTC46700.036.569213