FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox1060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox1060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8691327
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG245790.2827991628896255TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34650.053.0679746
CGTATGC35550.051.5303944
GTATGCC39550.046.58040645
ATGCCGT40400.045.68602847
TCGTATG42650.043.43739743
CTCGTAT46500.039.544242
TATCTCG45900.039.53544239
GCACACG54600.037.02742411
CACTAGC51000.036.9365231
TAGCTTA50650.036.7824234
TGCCGTC51100.036.11968248
GCCGTCT51200.036.11651649
CACACGT56500.035.6601312
TCACTAG52950.035.51109730
ACGTCTG58500.034.55912415
TCTCGTA54000.034.1160341
CACGTCT61600.032.87539314
ACACGTC62200.032.55882613
GTCACTA61400.031.18570929
AGTCACT63250.030.7644828
Produced by FastQC (version 0.11.2)