FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox10240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox10240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11740435
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG189510.16141650628788456RNA PCR Primer, Index 34 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32150.044.854944
TATGCCG32900.044.25163346
GTATGCC38300.038.19281445
TCGTATG37650.037.11116843
ATGCCGT38850.035.8757747
CTCGTAT44450.030.96818442
GCACACG58150.028.12132811
GCCGTCT49050.027.43067449
CACACGT61650.026.69281212
ACGTCTG62900.026.27271815
CATCTCG54900.025.8278239
TGCCGTC55400.024.97154848
GGCATCT57100.024.71186437
TCTCGTA56600.024.19861641
ACACGTC68100.024.0636313
CACGTCT67800.023.86479614
GTGTGCG19550.022.9404498
AGTCACC69750.022.7038628
AGCACAC72700.022.63536310
CAGTCAC71850.022.32820127
Produced by FastQC (version 0.11.2)