FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox10240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox10240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9860945
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG425940.43194643109762804RNA PCR Primer, Index 33 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57050.056.4206146
CGTATGC57300.056.23465344
CCAGGCG59750.053.69974533
GTATGCC61800.052.1957245
ATGCCGT63250.050.99914647
TCGTATG64550.050.0789443
CAGGCGA64300.049.73912434
CTCGTAT66700.046.75783542
ACCAGGC70450.045.69047532
GGCGATC67300.045.6752636
AGGCGAT69200.045.56807735
GCGATCT70100.043.85085337
TGCCGTC74800.043.35488548
GCCGTCT76150.042.40506449
GCACACG78000.042.01873411
TCTCGTA73750.041.91408541
CACACGT80300.040.85879512
ACGTCTG80400.040.59321615
ACACGTC81800.040.025213
CACGTCT86200.038.06200414