FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10222624
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG160430.1569362230284514TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29200.041.11595546
CGTATGC30450.039.881344
GTATGCC34650.034.9476445
ATGCCGT34750.034.84707347
TCGTATG37450.032.2426143
CTCGTAT43000.028.4019942
TGCCGTC43650.027.58386648
GCACACG46300.027.19743711
GCCGTCT44550.027.10405749
ACGTCTG48650.026.167615
CACACGT48700.026.14060612
ACACGTC48350.026.11589813
TCTCGTA49750.024.4791141
CATCTCG51100.024.10257339
CACGTCT53450.023.49469414
AGCACAC58350.021.93558110
AGTCACC59100.020.78178828
CGGGCGC8400.020.53577415
CAGTCAC62200.020.3560727
CGTCTGA63000.020.04291516
Produced by FastQC (version 0.11.2)