FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10585466
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG152430.14399932889114186TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28150.039.83093644
TATGCCG28150.039.3407146
GTATGCC33500.033.5728645
ATGCCGT34500.032.59988847
GTCACGC35000.032.52892329
TCGTATG36000.031.24141143
TATCTCG39600.028.14005539
AGTCACG41400.027.75029828
CACGCCA41400.027.66683431
GCACACG43300.027.249211
CGCCAAT41600.026.78729433
TCACGCC42900.026.53874630
CTCGTAT42700.026.33936542
ACGTCTG46100.025.6689915
GCCGTCT43650.025.6081649
TGCCGTC44750.025.28706248
GCCAATA44600.025.21740334
ACGCCAA45950.025.15237232
CACACGT48900.024.1991912
ACACGTC49600.023.6491113
Produced by FastQC (version 0.11.2)