FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox0240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox0240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13191834
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27050.036.47625744
TATGCCG28600.034.62002646
GTATGCC33850.029.7601945
TCGTATG32250.028.5622943
ATGCCGT37300.026.17514447
CGGGCGC21550.024.6544215
GGCGGGC22250.024.18925713
CTCGTAT39400.023.11634642
GCGGGCG23950.022.61581214
GCACACG53300.020.97212611
ACGTCTG55650.020.45832815
CACACGT55150.020.39388812
GCCGTCT47200.020.17337449
GTGTGCG18350.020.1176538
TCGGGCG30800.019.6027477
TGCCGTC51850.018.69687748
CGGCAAT41150.018.6143951
TAGCCCG29300.018.6039834
ACACGTC61000.018.32496313
ATCGGGC32750.018.2256286
Produced by FastQC (version 0.11.2)