FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ox0240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ox0240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12853202
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC11500.017.100215
GTGTGCG17750.014.7717168
CGCGTAT10150.014.2773061
GGCGGGC14550.013.51567513
CGGCAAT32000.013.2622791
GCGGGCG16150.012.17651814
CGCGGGG2902.8144821E-511.8977551
CGGGCAT16650.011.8120061
GACTGTC38950.011.5151664
ATCGGGC24650.011.1971466
CGTATGC15800.011.13590244
TAGCCCG18650.010.91437934
TCGGGCG23300.010.6609037
GGGAGAT68300.010.6592041
CTGACAT49800.010.5311851
CCGACTG34500.010.4002842
GTGCGAG26900.010.13181310
GGGAAAT59350.010.1155981
CGACTGT44450.09.9351063
TATGCCG18200.09.85691346