FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko6060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko6060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9211984
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA112340.1219498427266048TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA22050.036.7691435
TATGCCG22500.036.646348
CGTACGT23650.034.71959734
CGTATGC23850.034.1378446
CCGTACG25200.033.2686133
TACGTAA25450.031.99251736
TCGTATG28550.029.00114845
ATGCCGT29300.028.61218649
GTATGCC29350.028.44574547
ACGTAAT28900.028.29256637
CTCGTAT33250.024.48684144
GCCGTCT34100.024.38232251
ACGTCTG37050.024.02431115
TGCCGTC36300.023.28476550
ACCGTAC36050.023.255632
GCACACG38600.023.0594811
CGTAATC35950.022.840238
CACACGT40800.021.73175412
ACACGTC42850.020.85321813
TCTCGTA41450.019.72587243
Produced by FastQC (version 0.11.2)