FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko6060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko6060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10333030
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA183530.17761489127584068TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC31550.042.8644946
TATGCCG32550.041.653848
ATGCCGT36050.037.8968449
GTATGCC37800.035.8685547
GGCCTTA38050.035.63374736
TCGTATG37950.035.5447945
TATCTCG42550.031.62148941
GTCACGT43450.031.3647229
AGTCACG43950.031.243428
CTCGTAT43050.031.17378244
ACGTGGC44950.030.54832832
GCCTTAT44900.030.5047737
CACGTGG44850.030.4625931
CGTGGCC46500.029.82683433
TGCCGTC47400.029.11352250
GCCGTCT47400.028.82238651
TCACGTG48100.028.69135730
ACGTCTG49750.027.94621315
CACACGT49600.027.89134612
GCACACG49650.027.79377211
Produced by FastQC (version 0.11.2)