FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12063248
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG437640.36278786608714336RNA PCR Primer, Index 17 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC64000.052.77414344
TATGCCG64750.052.10958546
GTATGCC70550.048.11945
ATGCCGT70750.047.9829747
TCGTATG72100.046.9409943
TCACGTA74450.045.41315530
ACGTAGA77600.043.52506632
CTCGTAT77450.043.0750142
GTCACGT79200.042.86375429
AGTCACG79600.042.301828
CGTAGAG82450.040.92326733
TGCCGTC83650.040.70688648
ACGTCTG85100.040.2967415
GCACACG85400.040.15501411
GCCGTCT85250.039.94305449
CACGTAG84500.039.8894631
CACACGT88150.038.8633312
TCTCGTA86850.038.61150741
ACACGTC90950.037.59132413
CACGTCT94100.036.33280614
Produced by FastQC (version 0.11.2)