FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8692879
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG313650.36081256854029603TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC43800.054.58624333
TATGCCG44550.053.8203146
CGTATGC45700.052.4662744
GTATGCC48350.049.4476745
ATGCCGT48600.049.264347
TCGTATG49450.048.48753443
CTCGTAT52650.044.09895742
CCGTCCA55150.043.35226434
CACCCGT56250.042.6269131
TGCCGTC56400.042.5123248
ACCCGTC56750.042.31189332
GCCGTCT58500.040.92726549
CGTCCAT57050.040.51685735
TCACCCG59850.040.1786430
ACGTCTG61600.039.8201615
GCACACG62500.039.578411
GTCACCC61400.039.55768629
CACACGT64350.038.17271412
TCTCGTA60750.037.19688841
ACACGTC66000.037.16612213