FastQCFastQC Report
Thu 25 Jan 2018
HWNG7BGX3_n01_ko1060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWNG7BGX3_n01_ko1060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10382921
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA410130.39500445009646135TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC57750.054.54222546
TATGCCG57600.054.4446848
GTATGCC62350.050.29668447
TCGTATG64900.048.42701745
ATGCCGT64700.048.3101149
AGTCACG69100.045.4842128
GTCACGT69650.045.12547729
TCACGTG69800.044.92986330
CTCGTAT69300.043.85901344
CACGTGA72600.043.52926331
TGCCGTC73800.042.72714650
GCCGTCT74900.041.91560751
ACGTCTG78200.040.58776515
GCACACG80500.039.77211811
ACGTGAA80600.039.2939732
CACACGT82150.039.0152812
TCTCGTA78700.038.88347243
ACACGTC84650.037.713
AACGATC79900.037.6949938
TGAAACG85050.037.15689535
Produced by FastQC (version 0.11.2)